REF.: 111001200
Vendido e entregue por MPBIO
The GENECLEAN® kit simplifies the process of purifying DNA into three easy steps: Bind,Wash and Elute. Ethanol precipitation is never required and purified DNA is immediately ready for a wide variety of downstream applications. The GENECLEAN® Kit is used to purify fragments of DNA from 200 bp to 20 kb. The use of optional SPIN Filters allows isolation of fragments up to 300 kb.
The GENECLEAN® kit simplifies the process of purifying DNA into three easy steps: Bind,Wash and Elute. Ethanol precipitation is never required and purified DNA is immediately ready for a wide variety of downstream applications. The GENECLEAN® Kit is used to purify fragments of DNA from 200 bp to 20 kb. The use of optional SPIN Filters allows isolation of fragments up to 300 kb.
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Purify DNA from agarose gel| Desalting| Elimination of proteins from enzymatic reactions| Removal of primers and unincorporated nucleotides from enzymatic reactions| Separation of linearized from uncut vector| Isolation of PCR products away from genomic DNA
| SKU | 111001200 |
| Alternate Names | DNA Clean-Up Kit |
| Application Notes | The GENECLEAN® kit simplifies the process of purifying DNA into three easy steps: Bind,Wash and Elute. Ethanol precipitation is never required and purified DNA is immediately ready for a wide variety of downstream applications. The GENECLEAN® Kit is used to purify fragments of DNA from 200 bp to 20 kb. The use of optional SPIN Filters allows isolation of fragments up to 300 kb. |
| Base Catalog Number | 1001200 |
| Brand | GENECLEAN® |
| Components | NaI| New Wash Concentrate| Glassmilk™| BioFloat™ floating incubation rack |
| Format | Geneclean® slurry |
| Pack Size | 200 Preps |
| Personal Protective Equipment | Personal-protective-equipment: Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice. |
| pH | NAI pH Range: 7.0-7.4| Geneclean New Wash pH Range: 7.4 - 7.7 |
| Preparation Method | Pour contents of NEW Wash Concentrate (14 ml) into a bottle that accommodates at least 650 ml of liquid. Add 280 ml of distilled water and mix well. Add 310 ml of 100% (200 proof) ethanol and mix well. Label the prepared NEW Wash bottle with the label provided. Shake Glassmilk™ bottle until silica particles are in a uniform suspension. |
| Recommended Alternate | GENECLEAN® II Kit| GENECLEAN® III Kit| GENECLEAN® SPIN Kit| GENECLEAN® Turbo Kit |
| Safety Symbol | GHS05, GHS06 |
| Sample Source | Gels and Solutions |
| Sample Type | Enzymatic Reactions, Solutions, TAE buffered agarose gels |
| Sample Volume | Up to ~0.5 ml gel slice by volume or ~1.0 ml solution |
| Unit Definition | 1 μl of GLASSMILK™ will bind 1-2 μg of DNA in 4.5 M Nal in 5 minutes at room temperature. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
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